anti- g protein-coupled receptor kinase interacting arfgap 1 (git1) (Cell Signaling Technology Inc)
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anti- g protein-coupled receptor kinase interacting arfgap 1 (git1)
Anti G Protein Coupled Receptor Kinase Interacting Arfgap 1 (Git1), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+git1/git1+antibody/pmc03351456-80-15-31
Average 90 stars, based on 1 article reviews
Anti G Protein Coupled Receptor Kinase Interacting Arfgap 1 (Git1), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+git1/git1+antibody/pmc03351456-80-15-31
Average 90 stars, based on 1 article reviews
anti- g protein-coupled receptor kinase interacting arfgap 1 (git1) - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "VENNTURE–A Novel Venn Diagram Investigational Tool for Multiple Pharmacological Dataset Analysis"
Article Title: VENNTURE–A Novel Venn Diagram Investigational Tool for Multiple Pharmacological Dataset Analysis
Journal: PLoS ONE
doi: 10.1371/journal.pone.0036911
Figure Legend Snippet: (A) Proportional diagrams illustrating the number of specifically enriched and identified phosphoproteins, basally occurring, and induced by MeCh stimulation (10 nM to 100 µM) of control or CMP-treated cells. (B) Selective protein immunoprecipitation (IP) and determination of MeCh-induced G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1) serine and threonine phosphorylation, with antisera immunoblot (IB), in MeCh-stimulated control-state SH-SY5Y cells. The associated histogram (representing mean ± SEM data from three independent immunoprecipitations) indicates the extent of immunoprecipitated protein serine and threonine phosphorylation. Panels (C) and (D) indicate similar data from control-state cells for the MeCh-induced phosphorylation of G protein regulated inducer of neurite outgrowth 1 (GPRIN1) and p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1). (E) Selective protein immunoprecipitation and determination of MeCh-induced microtubule-associated protein 2 (MAP2) serine and threonine phosphorylation, with antisera immunoblot, in MeCh-stimulated CMP-state SH-SY5Y cells. Panels (F) and (G) indicate similar data from CMP-state cells for the MeCh-induced phosphorylation of reticulon-4 (RTN4) and GRB2-associated binding protein 2 (GAB2). Statistical analysis was performed on three independent experiments using GraphPad Prism version 5.02 with a Student’s t-test (p<0.05, *; p<0.01, **; p<0.001, ***).
Techniques Used: Immunoprecipitation, Western Blot, Binding Assay
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Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting protein (GIT1) (cat. no. 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Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting protein (GIT1) (cat. no. Article Title: Rho kinase collaborates with p21‐activated kinase to regulate actin polymerization and contraction in airway smooth muscle Article Snippet: Escherichia coli (Bluescript) transformed with these plasmids was grown in LB medium, and plasmids were purified by alkaline lysis with SDS using a purification kit from Qiagen Inc (Germantown, MD, USA).. Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting Transmission Assay:Article Title: Rho kinase collaborates with p21‐activated kinase to regulate actin polymerization and contraction in airway smooth muscle Article Snippet: Escherichia coli (Bluescript) transformed with these plasmids was grown in LB medium, and plasmids were purified by alkaline lysis with SDS using a purification kit from Qiagen Inc (Germantown, MD, USA).. Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting protein (GIT1) (cat. no. Article Title: Rho kinase collaborates with p21‐activated kinase to regulate actin polymerization and contraction in airway smooth muscle Article Snippet: Escherichia coli (Bluescript) transformed with these plasmids was grown in LB medium, and plasmids were purified by alkaline lysis with SDS using a purification kit from Qiagen Inc (Germantown, MD, USA).. Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting Generated:Article Title: Rho kinase collaborates with p21‐activated kinase to regulate actin polymerization and contraction in airway smooth muscle Article Snippet: Escherichia coli (Bluescript) transformed with these plasmids was grown in LB medium, and plasmids were purified by alkaline lysis with SDS using a purification kit from Qiagen Inc (Germantown, MD, USA).. Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting protein (GIT1) (cat. no. Article Title: Rho kinase collaborates with p21‐activated kinase to regulate actin polymerization and contraction in airway smooth muscle Article Snippet: Escherichia coli (Bluescript) transformed with these plasmids was grown in LB medium, and plasmids were purified by alkaline lysis with SDS using a purification kit from Qiagen Inc (Germantown, MD, USA).. Sources of antibodies are as follows: monoclonal mouse anti‐human paxillin (cat. no. 610569, BD Biosciences, San Jose, CA, USA), polyclonal rabbit anti‐human paxillin phospho‐tyrosine 118 (cat. no. 44‐722G, Thermo Fisher Scientific, Waltham, MA, USA), polyclonal rabbit anti‐human paxillin phospho‐serine 273 (cat. no. 44‐1028G, Thermo Fisher Scientific), mouse monoclonal anti‐human talin (cat. no. T3287, Sigma‐Aldrich), polyclonal rabbit anti‐human ROCK1 (cat. no. 4035T, Cell Signaling Technology, Danvers, MA, USA), polyclonal rabbit anti‐human Pak1 (cat. no. 2602, Cell Signaling Technology), polyclonal rabbit anti‐human phospho‐Pak Thr 423/402 (cat. no. 2601, Cell Signaling Technology), polyclonal rabbit anti‐human G‐protein‐coupled receptor kinase‐interacting |